Pr. Csaba Soti

Participant code: SE

Pr. Csaba Soti

Semmelweis University, School of Medicine, Department of Medical Chemistry,

Laboratory of Chaperone Molecules.

Budapest, P.O.Box 260; H-1444 Hungary.

Group’s experience:

Our group has a long-standing research experience in chaperone biochemistry (especially the 90 kDa molecular chaperone family) in healthy and disease, such as experimental diabetes, where we study oxidative protein folding and the role of chaperones. We routinely apply biophysical and biochemical methods, and various techniques of cell biology and molecular biology. Recently we have described oxidant-sensitive SH-groups as well as a second, reduction-sensitive ATP-binding site of the 90 kDa heat shock protein, Hsp90. Both findings shed new lights on the function of this chaperone. Moreover, these structural elements are likely to play a prominent role Hsp90-dependent processes during ageing and in changes of redox homeostasis (like chronic inflammation and T-cell activation). So far there are no data on chaperone-governed or -assisted processes, like changes in metallothionein, iNOS, PARP, telomerase folding in healthy adult, aged and in centenarian subjects. The combination of our expertise in in vitro and in vivo protein folding studies, Hsp90-dependent substrate maturation and in Hsp90-dependent signal transduction with that of the other groups will provide new vistas on the role of chaperones in the ageing process.

Personnel involved.

1. Dr. Csaba Soti, (M) responsible and scientific supervisor, biochemistry, cell biology
2. Dr. Gábor Nardai, (M) biochemistry and chaperone biochemistry
3. Mr. Amere Subbarao Sreedhar (M), graduate student, cell biology, molecular biology
4. Ms. Éva Kalmár, (F)graduate student, molecular biology
5. Katalin Mihály, (F) technician, cell biology
6. Post-doctoral or pre-doctoral fellow to be acquired, biochemistry and cell biology
7. N° 1 Technician fellowship to be acquired, biochemistry

Recent relevant publications.

1) Soti Cs, Csermely P: Molecular chaperones and the aging process. Biogerontology 2000; 1: 225-233
2) Soti Cs., Csermely P.: Chaperones come of age. Cell Stress Chaperones 2002; 7: 186-190
3) Soti Cs., Csermely P.: Chaperones and aging: their role in neurodegeneration and other civilizational diseases. Neurochem. International 2002; 41: 383-389
4) Nardai G., Csermely P., Soti Cs.: Chaperone function and chaperone overload in the aged. A preliminary analysis. Exp. Gerontol. 2002, 37: 1255-1260
5) Soti Cs., Sreedhar A.S., Csermely P. (2003) Apoptosis, necrosis and cellular senescence: chaperone occupancy as a potential switch. Aging Cell 2003, 2: 39-45.